Test Directory

Home Test Search Results STEC PCR
STEC PCR

Justification

The STEC-PCR assay can be confirmed by the detection of a Shiga toxin-producing Escherichia coli (STEC) in the patient’s stool sample. The test looks for the presence of ST-1 and/or ST-2 genes in stool cultures. The most common cause of HUS is STEC. STEC-HUS accounts for over 90 percent of cases of HUS in children in the US and it is one of the main causes of acute kidney injury in children under the age of three years.

STAT: < 48 hours (M-F)

RT-PCR

Draw Tube: Cary-Blair Transport Medium

Sample Type: Stool in Transport Medium

Specimen Requirements

Sample Type Volume Required Minimum Volume Stability
PREFERRED Stool in Transport Medium 1 scoop (~1 gram) of stool into Cary-Blair Transport Medium For pediatric minimum, please call (800) 566-3462. Room Temp.: 96 hours
ALTERNATIVE - - - -
REJECTION CRITERIA Sample contamination; sample compromised
SPECIAL INSTRUCTIONS -

General Information

METHODOLOGY RT-PCR
STAT TAT < 48 hours (M-F)
STAT TAT Performance > 90% of results released in 48 hours
ROUTINE TAT < 5 days (M-F)
ALTERNATIVE NAMES STEC HUS, STEC PCR, O157:H7
DESCRIPTION -
LIMITATIONS -
NORMAL RANGE Negative
ASSOCIATED TESTING -
REFERENCES -
SAMPLE REPORT Upon request
NEW YORK STATE APPROVED -

Test Codes

ORDER CODE -
CPT CODE 87045; 87046 x2
LOINC CODE 625-4