Home Test Search Results Plasminogen Activator Inhibitor Activity (PAI-1) Gene Sequencing
Plasminogen Activator Inhibitor Activity (PAI-1) Gene Sequencing

Justification

The SERPINE1 gene codes for plasminogen activator inhibitor 1 (PAI-1). Reductions in PAI-1 can lead to bleeding problems while elevated levels of PAI-1 may increase the risk of thrombotic events. Genetic variants can shift PAI-1 levels in either direction, depending on the nature of the variant.

STAT: < 48 hours (M-F)

NGS

Draw Tube: Purple Top

Sample Type: EDTA Whole Blood

DRAW KITS AVAILABLE

Specimen Requirements

Sample Type Volume Required Minimum Volume Stability
PREFERRED EDTA Whole Blood 3mL 1mL Room Temp.: 1 month
Refrigerated: 1 month
ALTERNATIVE Cheek swab 2 swab - Room Temp.: 1 month
Refrigerated: 1 month
REJECTION CRITERIA Sample contamination; sample compromised
SPECIAL INSTRUCTIONS -

General Information

METHODOLOGY NGS
STAT TAT < 48 hours (M-F)
STAT TAT Performance > 90% of results released in 48 hours
ROUTINE TAT < 5 days (M-F)
ALTERNATIVE NAMES SERPINE1 gene sequencing, PAI-1 sequencing
DESCRIPTION This test sequences the exons plus 5bp of the flanking introns of the SERPINE1 gene. Note, this sequencing assay does not cover the 4G/5G promoter polymorphism; this polymorphism has been associated with thrombotic risk but is also very common (25-50% of the normal population carry the risk allele). Sanger sequencing may be used to confirm variants as needed.
LIMITATIONS This test will not detect variants located outside of the targeted DNA regions. This test is not optimized to detect chimerism or somatic mosaicism. This test will detect small indels but may miss larger deletions or duplications. Balanced structural variants will not be detected unless specifically targeted by a custom PCR assay.
NORMAL RANGE Interpretation: Negative
ASSOCIATED TESTING -
REFERENCES -
SAMPLE REPORT Upon request
NEW YORK STATE APPROVED No

Test Codes

ORDER CODE P3377
CPT CODE 81479
LOINC CODE 60577-4