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Factor VIII (8) Activity

Justification

Factor VIII (anti-hemophilic factor) is present in the liver, spleen, kidneys, and lymphocytes. In the plasma, Factor VIII circulates in a complexed form with von Willebrand factor, attached by a non-covalent linkage. Hemophilia A is characterized by a congenital deficiency of Factor VIII, and inherited in an X-linked recessive manner. The severity of the disorder depends on the Factor VIII:C level: < 1% (severe hemophilia), 1-5% (moderate hemophilia), 5-25% (mild hemophilia). Deficiencies in Factor VIII may also be acquired secondary to other diseases such as liver disease, disseminated intravascular coagulation (DIC), or the development of a specific factor inhibitor. Factor VIII is an acute phase reactant and may be increased due to stress, thromboembolic complications, coronary atherosclerosis, renal failure, diabetes, and other conditions.

STAT: < 24 hours (7 days a week)

Clot-based

Draw Tube: Blue Top

Sample Type: Citrated Plasma

Specimen Requirements

Sample Type Volume Required Minimum Volume Stability
PREFERRED Citrated Plasma 1mL 0.5mL Frozen (-20C): 2 weeks
Frozen (-80C): 6 months
ALTERNATIVE - - - -
REJECTION CRITERIA Thawed in transit, refrozen or clotted sample
SPECIAL INSTRUCTIONS -

General Information

METHODOLOGY Clot-based
STAT TAT < 24 hours (7 days a week)
STAT TAT Performance > 90% of results released in 24 hours 7 days a week
ROUTINE TAT < 3 days (M-F)
ALTERNATIVE NAMES Anti-Hemophilic Factor, F8, FVIII, clottable F8
DESCRIPTION This is a clot-based assay for the determination of Factor VIII Activity level in citrated plasma. Patient sample at three different dilutions is mixed with Factor VIII Deficient plasma containing normal levels of all other factors, such that the resulting activated Partial Thromboplastin Time is dependent only on the level of Factor VIII present in the patient sample. This value is compared to a reference curve generated from calibrators with known levels of Factor VIII Activity.
LIMITATIONS -
NORMAL RANGE 50-149%
ASSOCIATED TESTING -
REFERENCES -
SAMPLE REPORT Upon request
NEW YORK STATE APPROVED No

Test Codes

ORDER CODE P3053
CPT CODE 85240
LOINC CODE 3209-4