Test Directory
ADAMTS13 Antibody
Justification
Specimen Requirements
| Sample Type | Volume Required | Minimum Volume | Stability | |
|---|---|---|---|---|
| PREFERRED | Citrated Plasma | 1mL | 0.5mL | Room Temp.: 7 days Refrigerated: 7 days Frozen (-20C): 2 weeks Frozen (-80C): 6 months |
| ALTERNATIVE | - | - | - | - |
| REJECTION CRITERIA | Thawed in transit if shipped frozen, refrozen, clotted sample |
| SPECIAL INSTRUCTIONS | Mark Saturday delivery on FedEx airbill if shipping on a Friday. |
General Information
| METHODOLOGY | ELISA |
| STAT TAT | < 48 hours (M-F) |
| STAT TAT Performance | > 90% of results released in 48 hours |
| ROUTINE TAT | < 5 days (M-F) |
| ALTERNATIVE NAMES | VWF-Cleaving Protease Antibody, ADAMTS-13 Antibody Titer |
| DESCRIPTION | Chromogenic ELISA for the quantitative detection of anti-ADAMTS13 antibodies. ADAMTS13 is an enzyme that specifically cleaves large von Willebrand Factor multimers, which induce platelet thrombus formation under high shear stress. If the activity of ADAMTS13 is lowered for some reason, unusually large VWF multimers may accumulate. |
| LIMITATIONS | - |
| NORMAL RANGE | < 12 U/mL |
| ASSOCIATED TESTING | ADAMTS13 Gene Sequencing; aHUS Genetic Panel; TMA-Complete Genetic Panel |
| REFERENCES | 1. Bethany, G et al. ADAMTS-13 activity and autoantibodies classes and subclasses as prognostic predictors in acquired thrombotic thrombocytopenic purpura. J Thromb Haemost. 2012;10:1556-1565. |
| SAMPLE REPORT | Upon request |
| NEW YORK STATE APPROVED | Yes |
Test Codes
| ORDER CODE | P3342 |
| CPT CODE | 83520 |
| LOINC CODE | 40824-5 |