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Factor IX (9) Activity

Justification

Factor IX (Christmas Factor) is synthesized by the liver and is vitamin K-dependent. Hemophilia B is characterized by a congenital deficiency of Factor IX, and inherited in an X-linked recessive manner. The severity of the disorder depends on the Factor IX level: <1% (severe hemophilia), 1-5% (moderate hemophilia), 5-25% (mild hemophilia). Deficiencies in Factor IX may also be acquired secondary to other diseases such as liver disease, disseminated intravascular coagulation (DIC), vitamin K deficiency, or the development of a specific factor inhibitor.

STAT: < 24 hours (7 days a week)

Clot-based

Draw Tube: Blue Top

Sample Type: Citrated Plasma

Specimen Requirements

Sample Type Volume Required Minimum Volume Stability
PREFERRED Citrated Plasma 1mL 0.5mL Room Temp.: 2 hours
Refrigerated: 2 hours
Frozen (-20C): 2 weeks
Frozen (-80C): 6 months
ALTERNATIVE - - - -
REJECTION CRITERIA Thawed in transit, refrozen or clotted sample
SPECIAL INSTRUCTIONS -

General Information

METHODOLOGY Clot-based
STAT TAT < 24 hours (7 days a week)
STAT TAT Performance > 90% of results released in 24 hours 7 days a week
ROUTINE TAT < 3 days (M-F)
ALTERNATIVE NAMES Christmas Factor, antihemophilic factor B, plasma thromboplastin component, F9, FIX
DESCRIPTION This is a clot-based assay for the determination of Factor IX Activity level in citrated plasma. Patient sample at three different dilutions is mixed with Factor IX Deficient plasma containing normal levels of all other factors, such that the resulting activated Partial Thromboplastin Time is dependent only on the level of Factor IX present in the patient sample. This value is compared to a reference curve generated from calibrators with known levels of Factor IX Activity.
LIMITATIONS -
NORMAL RANGE 88-150%
ASSOCIATED TESTING -
REFERENCES -
SAMPLE REPORT Upon request
NEW YORK STATE APPROVED -

Test Codes

ORDER CODE P3054
CPT CODE 85250
LOINC CODE 3187-2